Projects

Role of erythropoietin (Epo) in neuronal differentiation and neuroprotection after perinatal hypoxic injury

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Immature neurons (DCX, green) and mature neurons (Map2, red) 12 days in vitro (12 DIV)

 

 

  • Role of Epo in proliferation, cell death and differentiation, analyzed in vitro and in mutant mice overexpressing cerebral Epo and mice lacking the Epo receptor in neuronal stem cells (Nestin-cre;EpoRflox/flox)
  • Analysis of signal transduction pathways initiated by hypoxia and Epo
  • Evaluation of the effect of Epo on the postnatal maturation of GABAergic neurons
  • Analysis of Epo neuroprotection on the GABAergic system upon perinatal hypoxia

Neuroprotective role of erythropoietin (Epo) in perinatal white matter injury

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Oligodendrocytes forming myelin (MBP, red) around axons (NF-200, green) in the white matter early in postnatal development (P7)

 

 

  • Role of Epo in proliferation, differentiation and synaptic integration of oligodendrocyte precursor cells, analyzed in mutant mice overexpressing cerebral Epo and mice lacking the Epo receptor in oligodendrocyte progenitor cells (Sox10cre-EpoRflox/flox)
  • Evaluation of Epo’s effect on the maturation rate of oligodendrocytes and myelination
  • Analysis of Epo neuroprotection after perinatal white matter injury
  • Signaling pathways promoting oligodendrocyte maturation

Role of erythropoietin (Epo) in brain metabolism, the neurovascular unit and the blood-brain barrier (BBB)

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Blood vessels (CD31, red) surrounded by radial glia endfeet (dystroglycan,green) in the juvenile hippocampus (P21)

 

 

  • Role of Epo in angiogenesis and astrogenesis, analyzed in vitro and in vivo in mutant mice overexpressing cerebral Epo
  • Changes in astrocytic reactivity caused by Epo and perinatal hypoxia
  • Effect of Epo in brain mitochondrial respiration
  • Evaluation of Epo’s effect on cerebral blood volume (CBV) and cerebral blood flow (CBF) under normoxic and hypoxic conditions
  • Changes in the neurovascular unit caused by Epo under normoxic and hypoxic conditions
  • Effect of Epo on the blood brain barrier (BBB) after perinatal hypoxia

Techniques and Equipment

 

In vitro: rat primary neuron cultures, astrocyte and endothelial cell cultures

Immunohistochemistry: single and multiple labeling studies in tissue sections and cell cultures

Confocal laser scanning microscopy and epifluorescence microscopy

Electron microscopy; in situ hybridization; western blot

Stereotaxic surgery, intracerebral virus injections, EEG recordings

Electrophysiology (patch clamp)

High-resolution respirometry (Oroboro), plethismography, hypoxic chambers for in vitro and in vivo studies

Behaviour